Presentation Several members of the Calvert Laboratories staff recently authored a poster titled A Percutaneous Intrasalivary Gland Dosing Technique in Rats, and their work was presented November 913, 2008 at the 59th American Association for Laboratory Animal Science National Meeting in Indianapolis, IN. Employees who assisted with this authorship were Jaclyn G. Tontini, DVM, Laurie M. Serfilippi, VMD, DACLAM, Danielle R. Pallman, BS, CVT, Cesar V. Mujer, Ph.D., Charles B. Spainhour, VMD, Ph.D., DABT. Dr.Laurie Serfilippi had the honor of presenting the poster at the meeting. This technique was developed as a result of one of Calvert Labs client requests to developing a non-invasive and consistently repeatable dosing technique to deliver test particle to the submandibular salivary glands of rats. Since the company strives to personalize and optimizing client service, the Calvert team went to work to address this challenge and develop an appropriate technique. The first consideration in the quest to solve this puzzle was to use surgery and ultrasound to aid us in locating and localizing the glands for injection. However, due to equipment costs, invasiveness, and the need to dose multiple times, everyone decided to look into other options. During the process, the team found that if they anesthetized Sprague Dawley rats, placed them in dorsal recumbency, and extended their necks (which were shaved and prepared with alcohol), the glands would be better visualized under the skin. The technicians could then use tissue forceps to isolate each individual submandibular salivary gland, and then they could easily inject test article percutaneously into the glands parenchyma. To insure that this theory was something of value, the staff initially tried the procedure on training animals using Evans Blue dye to ensure that the glands themselves were actually being injecting. The success rate during the initial training process was around 90% but as the technicians became more proficient, the rate grew to around 95%. To further study the effects of this dosing procedure, staff members dosed a couple of studies using the technique. The study animals did well post-injection and showed no obvious adverse effects to the injections or anesthesia. The staff members further confirmed that the test article injections were placed in the proper location through findings on histopathology. As a result, the members of Calvert Labs now feel reasonably confident that this technique could be considered along with surgical exposure or ultrasound guidance as another option to deliver test article to the salivary glands in the rat model. The full poster may be viewed here: .calvertlabs../2009/01/calvert-labs-poster-presented-at-national-meeting/ About the Author: 相关的主题文章: